[Oink]

Your defense range should depend on villains range and the rake.

Since you didnt post any reads I assume that villain is unknown. With the high rake at 0.1/0.2, folding A4o will not affect your winrate a lot.

Against an aggresive stealer you should definetely defend with A4o against the button. In fact I would defend with my top 65% HU against a player who steal more than 35%.

About the hand. The bet/3-bet on the flop is spew. You dont have eq and he is not folding a better hand. The turn donk is silly as well. He is not going anywhere and your draw is weak. By the river your only chance of winning is betting, but I dont think he folds enough to make it worthwhile.

c/f flop. As played c/f turn. As played c/f river. You really messed this one up.