A3s TP and Nutflusdraw on the flop
 

PokerStars No-Limit Hold'em, $0.25 BB (6 handed) Poker Stars Converter Tool from FlopTurnRiver.com (Format: 2+2 Forums)

UTG ($41.10)
MP ($60)
CO ($23.80)
Button ($24.30)
SB ($5.70)
Hero ($25)

Preflop: Hero is BB with A[img]/images/graemlins/diamond.gif[/img], 3[img]/images/graemlins/diamond.gif[/img].
UTG calls $0.25, <font color="#CC3333">MP raises to $0.5</font>, CO calls $0.50, Button calls $0.50, SB calls $0.40, Hero calls $0.25, UTG calls $0.25.

Flop: ($3) K[img]/images/graemlins/diamond.gif[/img], 8[img]/images/graemlins/diamond.gif[/img], A[img]/images/graemlins/spade.gif[/img] <font color="#0000FF">(6 players)</font>
SB checks, <font color="#CC3333">Hero bets $2</font>, UTG folds, MP folds, <font color="#CC3333">CO raises to $6</font>, Button folds, SB folds, <font color="#CC3333">Hero raises to $24.5</font>, CO calls $17.30 (All-In).

Turn: ($49.60) 8[img]/images/graemlins/heart.gif[/img] <font color="#0000FF">(2 players, 1 all-in)</font>

River: ($49.60) 9[img]/images/graemlins/club.gif[/img] <font color="#0000FF">(2 players, 1 all-in)</font>

Final Pot: $49.60

Can I play this hand like this against an unknown villain?

Re: A3s TP and Nutflusdraw on the flop
 

Yes.

PF is close, getting so many callers. If it were less, I fold there. Playing draws OOP sucks.

Flop is standard. You have 9 outs to the nuts, 3 outs to a good hand (2P) and 2 outs to trips (which may or may not be good). This shove is +EV especially given fold equity.

Re: A3s TP and Nutflusdraw on the flop
 

Seems fine to me.

Re: A3s TP and Nutflusdraw on the flop
 

I really doubt there is any fold equity at this loose table...but shove in this spot seems standard.

I'm just wondering though, is this play negative EV in the long run because getting it in as only a 40% favorite or so with not enough dead money in the pot to cover for us seems to be a losing scenario. Like I said, there is not much fold equity.

Does anyone consider a flop call to CO's raise?