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Only if you find an ATM card that will allow a $1000 transaction. More likely you will have to break it up into two $500 withdrawls.

The $2.50 fee (per transaction) is from the payTru VISA Debit card side. The bank that actually owns the ATM machine is going to charge $2 (per transaction) or more also for using a card that doesn't belong to them.

So your $500 in, $1000 out transaction is going to cost you at least $28.50 in fees, if not $33 or more, if you have to do multiple withdrawls at the ATM.